Ex vivo expansion of limbal stem cells is affected by substrate properties
نویسندگان
چکیده
منابع مشابه
Ex vivo expansion of limbal stem cells is affected by substrate properties.
Limbal epithelial stem cells play a key role in the maintenance and regulation of the corneal surface. Damage or destruction of these cells results in vascularisation and corneal opacity. Subsequent limbal stem cell transplantation requires an ex vivo expansion step and preserving cells in an undifferentiated state remains vital. In this report we seek to control the phenotype of limbal epithel...
متن کاملEx vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures.
BACKGROUND/AIM Amniotic membrane (AM) transplantation effectively expands the remaining limbal epithelial stem cells in patients with partial limbal stem cell deficiency. The authors investigated whether this action could be produced ex vivo. METHODS The outgrowth rate on AM was compared among explants derived from human limbus, peripheral cornea, and central cornea. For outgrowth of human li...
متن کاملEx vivo expansion of corneal limbal epithelial/stem cells for corneal surface reconstruction.
PURPOSE The management of severe ocular surface disease due to limbal stem cell deficiency has changed dramatically. The concept of limbal stem cells, as the source of corneal epithelium revolutionised the therapeutic approach of ocular surface reconstruction. Deficiency of limbal stem cells results in blinding ocular surface diseases. Grafting viable limbal tissue, from either fellow healthy e...
متن کاملEx vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow
Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then different...
متن کاملVariations of the Normal Human Limbal Stem Cells Detected by In Vivo Confocal Microscopy
Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2012
ISSN: 1873-5061
DOI: 10.1016/j.scr.2012.01.001